The National Centre for Industry 4.0 is an open platform connecting innovation leaders from universities, companies, and other organizations, all working together to advance industry in the Czech Republic. The Centre creates a unique ecosystem of 60 partners and serves as a driving force behind the development of Industry 4.0, digitalization, and artificial intelligence in the country.The Centre represents the most significant technical universities, industrial enterprises, and key organizations such as the Confederation of Industry of the Czech Republic, the Czech Chamber of Commerce, and the Association of Small and Medium-Sized Enterprises of the Czech Republic. The National Centre for Industry 4.0 has ensures the effective transfer of know-how in the fields of Industry 4.0, digitalization, and artificial intelligence into industrial practice, acting as a bearer of technological visions. Through its expert activities and the implementation of technological projects, the Centre actively contributes to fulfilling these visions. The Centre educates, raises awareness about industrial technologies, and creates an environment for the digital transformation of industry and society. It has already helped hundreds of Czech companies navigate technological possibilities, set up digital strategies, and identify suitable development opportunities.The Centre fosters an environment that integrates Czech research and industry into the broader European infrastructure for advanced industrial manufacturing. This enables companies to develop and validate technological solutions while gaining access to strategic European initiatives such as EDIHs, AI-MATTERS TEF, EIT Manufacturing, and GAIA-X.